![]() ![]() Urea is light sensitive and can undergo autohydrolysis.When performing overnight tests from medium that contains peptone, the alkaline reaction may be due not to urease but to hydrolysis of peptone.Some organisms rapidly split urea ( Brucella and H.C: Cryptococcus neoformans /Corynebacterium spp.Note: Mnemonics to remember urease positive organisms: PUNCH (Similar mnemonic for oxidase- positive organism is PVNCH) Proteeae ( Proteus spp., Morganella morganii, and some Providencia stuartii strains).This is a highly sensitive and specific test. Urea breath test : A common noninvasive test to detect Helicobacter pylori also based on urease activity. ![]() neoformans may be based on rapid urease production, whereas Candida albicans do not. Rapid urease test is can be used to differentiate between the yeasts, Candida albicans, and Cryptococcus neoformans.Commercially available urease agar kits are also available. A positive urease test is considered the presence of Helicobacter pylori. This is done by placing a portion of crushed tissue biopsy material directly into urease broth. Urease test is used for the presumptive evidence of the presence of Helicobacter pyloriin tissue biopsy material. ![]()
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